normally i get shoots from basilcum directly when i put explants  in MS medium with  hormones , 

now i m trying to introduce new gene to basilicum using  two differents agrobacterium , i have protocol i tried it but it doesn t work , i tried to modify it arround 5 times but no results till now, ( the leaves become dried , i keep changing the medium every 2 weeks but at the end i get only some died brown calli ...!!