Can Cryo-Sem be used for small protein 3D structure building?

Hello, I have a small purified protein, 15kDa. Iam seeking some technical service to build its 3D structure by Cryo -Sem, or NMR. However, I was told by some technicians that Cryo-Sem is only...

12 January 2021 5,072 3 View

Why did I get negative peaks in HPLC with a UV detector and a SEC column?

Hello, Today, when I analyzed my nectar sample (containing plant secondary metabolites, amino acids and proteins) using a HPLC with a UV detector (220nm) and a SEC column, NaOAC buffer as mobile...

17 December 2019 8,863 8 View

How to read the protein MW determined by LC/MS?

Dear all, Recently, I isolated a protein by LC (IEC and GFC) which showed one band on SDS-gel. Then, I sent it to determine its accurate MW using LC/MS. The brief result is attached which showed...

20 August 2019 2,730 4 View

What were the chief reforms in the U.S. Gilded Age and the Progressive Era?

From the Gilded Age to the Progressive Era. What were the chief problems, and what new federal legislation was passed to meet those problems? What did these problems have to do with the rapid...

18 June 2019 6,209 79 View

What is oligarchy? Is there a present danger of oligarchy?

I quote from the Encyclopedia Britannica, Aristotle used the term oligarchia to designate the rule of the few when it was exercised not by the best but by bad men unjustly. In this sense,...

10 July 2018 7,369 91 View

I have no raw data, but the peak list and search result, can I still submit the data into PRIDE?

Dear Colleagues, I am required to submit my proteomics MS data into PRIDE. But when I checked my data, I found I only have the peak list (txt) and the search result (html) but no so called RAW...

23 May 2018 657 5 View

How to track proteins in plants?

Dear colleagues, I detected some proteins in a tissue but the gene mostly expressed in another tissue. I guess the protein might be transported from the original tissue. How can I prove it or how...

11 February 2018 9,252 4 View

How to purify FAD-dependent enzyme?

Dear Colleagues I am trying to purify a FAD-binding protein. Is there a reliable method I can use to purity such proteins? Best Zha

13 April 2017 1,190 8 View

Why there are extra bands on gRNA template PCR results?

02 March 2017 6,498 2 View

How to do geometry optimization of coordination complex using Materials Studio?

Now I'm trying to simulate the UV-Vis spectra using the "VAMP" module in Materials Studio. I found a problem when doing the geometry optimization. For a simple complex, copper hexahydrate ion, due...

19 December 2016 4,083 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Is this moth a Diatraea saccharalis?

The caterpillars of this moth were found in stems of Echinochloa polystachia grass as stem borers. The length of the caterpillars are something about 1.7 cm. I have compared images from the...

24 February 2021 309 3 View

Are these moths Spodoptera frugiperda?

The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...

24 February 2021 7,785 5 View

How to obtain free lipids starting from concentration over the CMC?

I need to work with free lipids at increasing concentration (50%, 60% of the CMC ect.). I'm now working with POPC and POPG. To reach these concentrations I have to start from concentration over...

17 February 2021 1,676 3 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View