8 Questions 23 Answers 0 Followers
Questions related from H.G. Zha
Hello, I have a small purified protein, 15kDa. Iam seeking some technical service to build its 3D structure by Cryo -Sem, or NMR. However, I was told by some technicians that Cryo-Sem is only...
13 January 2021 5,205 3 View
Dear All, Can adult butterfly or moth feed on solid food, such as pollen? It is known that nectar mainly contains sugar but very low level proteins, lipids. If a butterfly only feeds on nectar,...
30 March 2020 6,340 3 View
Hello, Today, when I analyzed my nectar sample (containing plant secondary metabolites, amino acids and proteins) using a HPLC with a UV detector (220nm) and a SEC column, NaOAC buffer as mobile...
18 December 2019 8,993 8 View
Dear all, Recently, I isolated a protein by LC (IEC and GFC) which showed one band on SDS-gel. Then, I sent it to determine its accurate MW using LC/MS. The brief result is attached which showed...
21 August 2019 2,846 4 View
Dear Colleagues, I am required to submit my proteomics MS data into PRIDE. But when I checked my data, I found I only have the peak list (txt) and the search result (html) but no so called RAW...
24 May 2018 794 5 View
Dear colleagues, I detected some proteins in a tissue but the gene mostly expressed in another tissue. I guess the protein might be transported from the original tissue. How can I prove it or how...
12 February 2018 9,374 4 View
Dear Colleagues I am trying to purify a FAD-binding protein. Is there a reliable method I can use to purity such proteins? Best Zha
14 April 2017 1,308 8 View
Dear colleagues, My colleague and I cloned a gene from sword bean which is mainly expressed in flower. After I did blast using the protein's amino acid sequence, I found there are just 250...
23 May 2016 7,819 9 View
