Hello, I would like to ask you a question. Recently, I injected 100ug OVA subcutaneously into C57BL/6 mice and conducted enhanced immunization on the seventh day. On the 21st day, cells from the spleen and inguin lymph nodes of mice were taken and CD3, CD4 and CD8 antibodies were detected to detect T cells. The ratio did not increase. I tried several times and got the same result. I did not know what the problem was. How many times can OVA subcutaneous injection cause obvious T cell proliferation and detection?