I am investigating the urease activity of S.pasteurii. Na+ and Cl- have not any influence on its urease activity, according to literature. Are there any other points which should be considered as a result of pH adjustment by NaOH and HCl?
No, adjusting the pH of culture media with NaOH or HCl con not influence on performance of bacteria
It should not matter whether you use NaCl or HCl as long as you use one of them! but If you adjust pH with NaOH and over do it do not use HCl to fix it (and vice versa) because you will create a salt and this may influence you media conditions and bacterial growth.
I used to adjust hte pH using HCL or NaOH without any effect on bacterial growth. I agree with Urbanek, NOT to fix the pH using both solution in order to avoid salt formation.
I would like to add a point with the above two suggestion, first you check the pH of culture media then you decide which one to opt. As Dr.Salman said there is a chances of formation of salt when you use both acid(HCl) and alkali(NaOH).
Pardon me: Isn't - if liquid - always a "buffered" solution providing a certain pH for growth of bacteria? (cf. also http://www.microrao.com/micronotes/culture_media.pdf, or http://textbookofbacteriology.net/nutgro_3.html) .
So, if you add NaOH to the buffer(ed)-culture medium, the addition of base will be "buffered" to the (presuming different buffer capacities). The same to the opposite side adding acid.... Have I missed here something ?
Hello, adjusting pH doesn't influence the bacterial growth on the culture medium.
Can we use NaOH to increase ph of culture media for lactic acid bacteria
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