Hi there, I have read that some (but not all) lentiviral transfer plasmids can be used in transient transfections to achieve transgene expression and those that can are primarily third-generation constructs. I would like to know if the pLVX vector is considered a third-generation construct and can be transiently transfected in cells.
If your gene of interest is expressed via its own promoter (e.g. CMV, EF1a, etc) then you can always use the lentiviral plasmid in transient transfections, regardless of the lentiviral vector generation.
The above answers are correct, but one more thing to add/note....keep in mind that if using 3rd gen vectors for transient expression, the far upstream promoter will also be active, and if you have a tissue-specific or other sensitive promoter expressing your construct, you gene of interest will also be under the upstream promoter.
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