Alejandro Correa Sáez

6 Questions 1 Answers 0 Followers

Questions related from Alejandro Correa Sáez

Can you use psPAX2 and pMD2G plasmids to produce viral particles to encapsulate a pLVX-M-Puro vector?

Hi there, I have transfected these two plasmids into 293T packaging cells to encapsulate a pLVX-M-Puro coding plasmid. I had already validated the stability of the pLVX-M-Puro insert by transient...

07 June 2024 8,246 5 View

Subcellular Fractionation to obtain ER fraction?

Hi there, I am planning an experiment to isolate the ER protein fraction from a cell culture. I have found a few commercially available kits, such as...

08 April 2024 2,971 0 View

Low 260/230 ratio after primer resuspension?

Hi there, I want to ask whether a ratio of 260/230 of 1,55 is considered low for primer after its resuspension. Thanks in advance

31 May 2023 1,670 3 View

Can a lentiviral vector (pLVX; Addgene 6108) plasmid be transiently transfected in cells to express the instert?

Hi there, I have read that some (but not all) lentiviral transfer plasmids can be used in transient transfections to achieve transgene expression and those that can are primarily third-generation...

28 February 2023 6,177 2 View

Can anyone recomend me an updated protein-ubiquitination database?

I want access to a database where proteins can be searched and the presence of ubiquitination and other parameters (ub link, mono/poly/branched ubi...)

21 December 2022 2,305 3 View

Is the precursor area of phosphorylated peptides a usefull tool to study the amount of phosphorylation between two conditions?

Hello everyone, Our group recently performed MS/MS spectrometry to study the possible alterations of a stimulus in specific protein phosphorylation. After obtaining the results we in fact...

11 May 2020 2,709 0 View