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Questions related from Alejandro Correa Sáez
Hi there, I have transfected these two plasmids into 293T packaging cells to encapsulate a pLVX-M-Puro coding plasmid. I had already validated the stability of the pLVX-M-Puro insert by transient...
07 June 2024 8,409 5 View
Hi there, I am planning an experiment to isolate the ER protein fraction from a cell culture. I have found a few commercially available kits, such as...
08 April 2024 3,069 0 View
Hi there, I want to ask whether a ratio of 260/230 of 1,55 is considered low for primer after its resuspension. Thanks in advance
31 May 2023 1,720 3 View
Hi there, I have read that some (but not all) lentiviral transfer plasmids can be used in transient transfections to achieve transgene expression and those that can are primarily third-generation...
28 February 2023 6,228 2 View
I want access to a database where proteins can be searched and the presence of ubiquitination and other parameters (ub link, mono/poly/branched ubi...)
21 December 2022 2,351 3 View
Hello everyone, We have predicted a docking structure of two different proteins and we pursued to determine the occurrence of alteration in the stability after punctual mutations. Thanks in advance
23 June 2020 6,328 3 View
Hello everyone, Our group recently performed MS/MS spectrometry to study the possible alterations of a stimulus in specific protein phosphorylation. After obtaining the results we in fact...
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