What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

Which are the appropriate standard in TLC as well as in HPLC ?

I have ethyle acetate extracts of photorhbdus bacteria which need to be identified but I am confused, when we are running TLC or HPLC to detect secondary metabolites, then d'nt know what to use...

19 January 2021 7,173 3 View

Where I could find a phospho-CPT1A antibody ?

I started working on CPT1A enzyme and particularly interested in the post-translational modifications of CPT1A. So I was looking for phospho-CPT1A antibody and couldn't find any. Can anybody tell...

09 September 2020 1,127 3 View

Has anyone tried electrospinning a polymer with higher concentration of salt (LiCl preferred)?

I am trying to electrospin polymer along with higher concentration of salt (more than 2% of salt). What might be the suitable polymer and suitable set up parameters?

30 July 2020 9,256 5 View

How to find out probability density function for an arbitrary set of data?

Dear All Firstly, I saw that probability density function is used in meteorology for comparison. For example, if I have two time series of same meteorological parameter from two different sources....

14 February 2020 797 1 View

Please suggest best process for N-methyl pyrrole synthesis ?

I need N-methylpyrrole,I was trid pyrrole reaction with Methyl iodide, and Dimethyl Sulphate,methanol/Acid not given good yields. so please suggest best process for N-methyl pyrrole synthesis

08 February 2020 1,226 3 View

I am facing in post processing of analysis of muffler on Ansys. How should I ensure that my muffler is not producing more then 110 db of sound ?

I am designing a muffler for my fsae car and I'm using KTM 390 engine. I did a flow analysis on muffler on ansys and plotted a transmission loss plot. But I don't know how to interpret it as I...

04 February 2020 6,713 1 View

Query regarding Index of Agreement Calculation?

Dear All This is Dr. Suman from IIT Bombay, India. Recently, I was evaluating model performance using Index of Agreement (Mbienda et al., 2017). IOA is a skill metrics used for performance...

03 August 2019 1,331 1 View

How can I prove the existence results of coupled non-linear ordinary differential equation using topological shooting argument?

I have read some papers regarding my question but i can't use these method always.

03 May 2019 3,329 3 View

How to calculate the % of segregation of concrete, which equipment should use to get accurate result ??

hello, i am currently working on ultra-lightweight self-compacting concrete, i need suggestion for measuring the % of segregation and bleeding. which machine/ equipment should use. also i want to...

02 April 2019 7,406 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View