Can 8E5 cells be cultured in DMEM media?

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When we measure capacitance with an LCR meter in series or parallel mode, what do series or parallel mean in this context?

How does an LCR meter works? When we measure capacitance with an LCR meter in series or parallel mode, what do series or parallel mean in this context? What components are in series or parallel?

16 May 2024 823 0 View

Which media is used for culturing batrachospermales ?

I have a fresh water red algae. Which media will be suitable to grow it ?

26 February 2024 8,391 1 View

How to prepare for Assistant Professor in History ? Kindly suggest questions liked to be asked ?

How to tackle with questions?? is it same in all universities???? please give your suggestions and make it a healthy discussion.

12 February 2024 7,512 1 View

How can we obtain daily building energy consumption data for a home?

We are currently in the process of developing a model to predict the energy consumption of a building using machine learning

10 February 2024 7,928 4 View

Which scopus or SCI indexed journal is accepting Checklist of plant?

Which Scopus or SCI indexed journal is accepting Checklist of plant. Please help me.

19 October 2023 4,553 0 View

Limit of Free Fattyacids in Emulsion samples (O/W) ?

Dear all, Can you please suggest me how to calculate the Limit of Free Fatty acids in Emulsion samples (O/W) by using titrimetric procedures.

29 August 2023 1,961 1 View

HRMS DATA analyzation of plant leaves extract compound?

Hello everyone, I want to ask the data interpretation of HRMS of plant leaves extract compound. Which compound of that plant should be consider whose value is given in form of relative abundance...

06 July 2023 288 0 View

Any idea about the compositon of anti fluorescence quenching reagent?

I want to know the composition of anti fluorescence quenching reagent,how can i make it .

18 May 2023 8,965 1 View

How do I make a LAMMPS input file from CHARMM's topology and parameter file?

We are making a topology file using the 'perl' command for Charmm2lammps conversion. We created master file by adding parameter file of lipid, carbohydrades, protein & cgenff. In that case,...

02 May 2023 1,951 0 View

Which sequence to select primers for a two-step RT-qPCR - mRNA sequence or cDNA sequence?

To perform a two-step RT-qPCR (starting material is RNA, then reversed transcribed to cDNA followed by cDNA amplification), do we have to design the sequence-specific primers for cDNA...

23 January 2023 6,441 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View