Any idea about the compositon of anti fluorescence quenching reagent?

19 May 2023 1 9K Report

I want to know the composition of anti fluorescence quenching reagent,how can i make it .

According to the Safety Data Sheet for the anti-fade product from Sigma-Aldrich (catalog number A7114), the solution contains 0.1% p-phenylenediamine dihydrochloride in glycerine.

https://www.sigmaaldrich.com/US/en/sds/mm/s7114

Badges
Science method

More Suman Arya's questions See All

Shortage Cost Hypothesis?

What can be a hypothesis related to the Stock out Cost or Shortage Cost?

29 July 2024 9,259 2 View

When we measure capacitance with an LCR meter in series or parallel mode, what do series or parallel mean in this context?

How does an LCR meter works? When we measure capacitance with an LCR meter in series or parallel mode, what do series or parallel mean in this context? What components are in series or parallel?

16 May 2024 823 0 View

How to determine the exact quantity of elements present in the biomass ?

I want to know for doing EDX analysis to examine the elements present in the biomass, should I do the first pyrolysis or directly do the powder sample analysis? which one is the most favorable?...

16 May 2024 7,883 2 View

Hi, I am looking for a computation tool to predict the formation/ of organic compounds when we heat different plants in a controlled combustion?

I am working on a project where we are looking for a computational tool to explore the occurrence of combustion products (organic molecules) when heating different kinds of plants in a controlled...

14 May 2024 561 1 View

How to read reduction potential from cyclic voltammetry plot for reduction peak and how to use this potential for calculating ERHE potential?

I want to interpret the data for the cathode catalyst analysis from the cyclic voltammetry curve therefore I want to know how to read the reduction potential for the oxygen reduction reaction. And...

01 April 2024 7,363 1 View

How to select geographical location of samples (defined sample size) for a primary demographic survey?

The primary survey is meant to analyse the social vulnerability status of the population, where sample size is defined at taluk level of the district (study area). Considering factors like...

05 March 2024 6,919 5 View

Which media is used for culturing batrachospermales ?

I have a fresh water red algae. Which media will be suitable to grow it ?

26 February 2024 8,391 1 View

Conductive Ink using CNT/ graphene/ nanoparticles?

Hii, Can anyone please suggest articles for preparation of conductive ink using CNTs/graphene/ nanoparticles, etc.?

22 February 2024 7,694 2 View

How to prepare for Assistant Professor in History ? Kindly suggest questions liked to be asked ?

How to tackle with questions?? is it same in all universities???? please give your suggestions and make it a healthy discussion.

12 February 2024 7,511 1 View

How can we obtain daily building energy consumption data for a home?

We are currently in the process of developing a model to predict the energy consumption of a building using machine learning

10 February 2024 7,928 4 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,842 0 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View