For a Free Thiol assay, I wanted to used DTNB and a reaction buffer. But since some materials for making reaction buffer is not available. I wanted to use SDS as reaction buffer.
Hi Santhosh,
2% SDS has been mentioned clearly as a reaction buffer in Pierce protocol.
Follow the link
http://www.piercenet.com/instructions/2160311.pdf
I want to know why the PLGA nanoparticle prepared by double emulsion method is not solubilizing in water or PBS. I have used 100 mg of PLGA in DCM and mixed with 7 mg of protein (O/W1) and...
05 June 2015 9,803 5 View
I wish to know how to use DIPEA or N,N-Diisopropylethylamine or Hünig's base for deprotonation of primary amine groups in polyethylenimine. Is it added based on molar ratio or weight ratio?
31 December 2014 545 2 View
I wish to know the amount of carboxyl groups in graphene oxide for a conjugation experiment. Is there any technique like acid base titration? If so can anyone provide a protocol for that?
09 October 2014 792 3 View
I have prepare thiolated PEI and I want to know the degree of thiolation. So I did the ellman's test with cysteine as the standard. Now how to calculate the degree of thiolation from this...
05 June 2014 1,556 1 View
Ninhydrin is used for testing the primary, secondary, and tertiary amines of synthetic cationic polymer or protein. Is the copper II acetate test only meant for primary and secondary amines in the...
04 May 2014 3,542 1 View
I have YOYO-1 dye and I want to add dye at 5:1 ratio. And I am using a plasmid of length 5411 bp. Please tell me how to calculate this ratio to get a volume of dye to be added to DNA of 1 microgram.
01 February 2014 1,589 2 View
I need to conduct a FRET analysis with labelled plasmid DNA and I am using YOYO-1 dye. I need to remove the free dye from the solution and get only the labelled DNA. Is there any technique with...
31 December 2013 8,603 1 View
If I want to just look at the RNA integrity (of RNA stored in -80 for more than a year), should I go for denaturing or non-denaturing electrophoresis? I've seen most people use formaldehyde gels...
02 March 2013 6,342 16 View
I have a control cell sample and drug treated cell sample in my experiment. I want to know which sample to be taken for creating a standard curve in relative real time PCR? Should I use the...
02 March 2013 4,008 0 View
Using the 2012b version of MATLAB, I wish to know how to import the data and analyse the data for normalization, fold change analyses and background correction.
11 December 2012 9,627 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View