20 Questions 31 Answers 0 Followers
Questions related from Santhosh Kalash
I want to know why the PLGA nanoparticle prepared by double emulsion method is not solubilizing in water or PBS. I have used 100 mg of PLGA in DCM and mixed with 7 mg of protein (O/W1) and...
06 June 2015 9,776 5 View
I wish to know how to use DIPEA or N,N-Diisopropylethylamine or Hünig's base for deprotonation of primary amine groups in polyethylenimine. Is it added based on molar ratio or weight ratio?
01 January 2015 523 2 View
I wish to know the amount of carboxyl groups in graphene oxide for a conjugation experiment. Is there any technique like acid base titration? If so can anyone provide a protocol for that?
10 October 2014 768 3 View
I have prepare thiolated PEI and I want to know the degree of thiolation. So I did the ellman's test with cysteine as the standard. Now how to calculate the degree of thiolation from this...
06 June 2014 1,523 1 View
Ninhydrin is used for testing the primary, secondary, and tertiary amines of synthetic cationic polymer or protein. Is the copper II acetate test only meant for primary and secondary amines in the...
05 May 2014 3,521 1 View
I have YOYO-1 dye and I want to add dye at 5:1 ratio. And I am using a plasmid of length 5411 bp. Please tell me how to calculate this ratio to get a volume of dye to be added to DNA of 1 microgram.
02 February 2014 1,559 2 View
For a Free Thiol assay, I wanted to used DTNB and a reaction buffer. But since some materials for making reaction buffer is not available. I wanted to use SDS as reaction buffer.
01 January 2014 3,984 1 View
I need to conduct a FRET analysis with labelled plasmid DNA and I am using YOYO-1 dye. I need to remove the free dye from the solution and get only the labelled DNA. Is there any technique with...
01 January 2014 8,572 1 View
If I want to just look at the RNA integrity (of RNA stored in -80 for more than a year), should I go for denaturing or non-denaturing electrophoresis? I've seen most people use formaldehyde gels...
03 March 2013 6,315 16 View
I have a control cell sample and drug treated cell sample in my experiment. I want to know which sample to be taken for creating a standard curve in relative real time PCR? Should I use the...
03 March 2013 3,984 0 View
Using the 2012b version of MATLAB, I wish to know how to import the data and analyse the data for normalization, fold change analyses and background correction.
12 December 2012 9,603 1 View
Are the parameters same as that for normal PCR? Please explain in detail.
09 September 2012 7,478 17 View
I am using an adherent cancer cell line and I need a whole cell lysis buffer for protein isolation. Can anyone tell the composition of the whole cell lysis buffer?
09 September 2012 6,813 6 View
Can anyone tell me how to create a heatmap using circos software? I also wish to know the input format to be fed in the software. Anyone who has the knowledge on Perl can really help me in...
09 September 2012 7,831 7 View
Appropriate concentrations of Penicillin and Streptomycin are used as antibiotics to prevent bacterial contamination in cell culture. Penicillin basically inhibits the peptidoglycan synthesis in...
07 July 2012 1,340 0 View
A question which still not properly answered by anyone.
08 August 2011 6,010 6 View
Hi friends, How to make a model of small molecules which could able to fit and activate a receptor? Is there any softwares or instruments? please explain me in brief.......
07 July 2011 6,707 2 View
Dear friends, There has been a debate between genetic disease and environmental relationship.. It tells us that nature is responsible for the genetic disease we are acquiring from generation to...
05 May 2011 778 2 View
What is the possibility that GM crops will be the main element for second green revolution in India?
05 May 2011 5,586 9 View
dear friends, In this forum we will talk on the future aspects of biotechnology and how it will be by the year 2050? I had strong thought over creation of regeneration medicines using nano...
04 April 2011 8,789 13 View
