How do I troubleshoot this solubility problem of PLGA nanoparticle loaded with protein and prepared by Double emulsion method?

05 June 2015 9,706 5 View

How can one use DIPEA for deprotonation of primary amine groups in polyethylenimine for protein conjugation?

31 December 2014 415 2 View

How can I calculate the degree of thiolation of a material containing free thiol group using Ellman's test?

I have prepare thiolated PEI and I want to know the degree of thiolation. So I did the ellman's test with cysteine as the standard. Now how to calculate the degree of thiolation from this...

05 June 2014 1,423 1 View

Is a copper II acetate test meant for checking the primary and secondary amine groups in a polymer?

Ninhydrin is used for testing the primary, secondary, and tertiary amines of synthetic cationic polymer or protein. Is the copper II acetate test only meant for primary and secondary amines in the...

04 May 2014 3,452 1 View

How can I calculate the DNA bp: dye ratio?

I have YOYO-1 dye and I want to add dye at 5:1 ratio. And I am using a plasmid of length 5411 bp. Please tell me how to calculate this ratio to get a volume of dye to be added to DNA of 1 microgram.

01 February 2014 1,444 2 View

Is there a way to separate free dye from fluorescently Labelled DNA?

I need to conduct a FRET analysis with labelled plasmid DNA and I am using YOYO-1 dye. I need to remove the free dye from the solution and get only the labelled DNA. Is there any technique with...

31 December 2013 8,505 1 View

Can 2% SDS be used as a reaction buffer for Ellman's test?

For a Free Thiol assay, I wanted to used DTNB and a reaction buffer. But since some materials for making reaction buffer is not available. I wanted to use SDS as reaction buffer.

31 December 2013 3,900 1 View

RNA gel electrophoresis - denaturing or non-denaturing??

If I want to just look at the RNA integrity (of RNA stored in -80 for more than a year), should I go for denaturing or non-denaturing electrophoresis? I've seen most people use formaldehyde gels...

02 March 2013 6,224 16 View

Standard curve for relative real time PCR

I have a control cell sample and drug treated cell sample in my experiment. I want to know which sample to be taken for creating a standard curve in relative real time PCR? Should I use the...

02 March 2013 3,916 0 View

How to analyse agilent microarray data using MATLAB?

Using the 2012b version of MATLAB, I wish to know how to import the data and analyse the data for normalization, fold change analyses and background correction.

11 December 2012 9,519 1 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View