Peptide lost during reverse phase HPLC?

Hello; I've dissolved my synthetic peptide in pure acetic acid and tried to purify it with reverse phase HPLC. I got weak peaks during analysis, however I injected at least 5 mg of my peptide!...

02 March 2019 9,491 5 View

How to calculate Kd with flowcytometric analysis?

Hello; I want to calculate Kd value for a FITC-conjugated peptide which specifically binds to a cell receptor. I've studied some protocols, but still confused! Does anyone have any experience or...

11 December 2018 7,119 2 View

Any protocol for solution based ELISA?

Hello; I've isolated peptides against PDL-1 from a phage library, but I can't confirm their binding to target in a solid based ELISA assay. So I want to check whether if I can get a positive...

04 May 2018 1,971 3 View

What is the easiest way to remove SDS following protein purification from gel?

I've purified my recombinant protein from SDS polyacrylamide gel and now I need to remove SDS for further work. Is it possible to do a buffer exchange on amicon filters? could anyone suggest any...

01 February 2017 1,165 4 View

Does anyone know how to justify the sample size in animal studies?

Hi there; I want to investigate anti tumor effects of an antagonist in murine models and I need to know how to determine number of animals in each group such that the results would be...

11 December 2016 1,166 9 View

What does the last number in cell line name mean?

Hi there; Does anyone one know what's the difference for example between SK-MEL-28 and SK-MEL-37? what does that number mean?

11 December 2016 3,576 0 View

Is there any way to induce tumorogenesis in murine models using human cell lines?

Hi there; I need to evaluate an anti cancer vaccine against a human protein in murine models.So I should use human cell lines. Is there any convenient way to induce tumorogenesis in murine models...

09 October 2016 1,859 3 View

Is there any convinient assay for in vivo gene expression evaluation?

Hi there; I want to needle inject Balb/C mouse with a plasmid coding for a specific gene and also GFP as a reporter and I need a method for in vivo gene expression assay! Is there any special kit...

06 July 2016 3,577 3 View

Why it's optimal for industrial microorganisms to grow in high temperature?

Hi there; I've read somewhere that growing in high temperatures is an advantage for industrial microorganisms; but unfortunately I couldn't find any reason for this in related articles! Is there...

04 May 2016 3,022 5 View

How can I detect secretary GFP-fused proteins in DMEM supernatant?

I've transfected HEK293-T cells with plasmid expressing for a secretary GFP-fused protein. I would like to know is there any method rather than ELISA test to detect my protein in supernatant using...

04 May 2015 4,653 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 324 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View