Calculating Kcat from Specific activity?

Erome Daniel Hankore @Erome_Daniel_Hankore

06 June 2017 0 3K Report

[E]: 10 ug/mL

Vmax: 0.098 U/mg

Protein mw: 128 kDa

Reaction volume: 150 uL

I'm having trouble getting to S-1 units for Kcat determination.

Kcat = Vmax/[E] so I should divide 0.098 U/mg by 10 ug/mL of enzyme concentration right?

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