Ca some one suggest the best protocol to purify centrosomes without any nuclear envelope/ER contamination.?

Karthigeyan Dhanasekaran @Karthigeyan-Dhanasekaran

12 July 2017 0 10K Report

I have been following the Tim Stearns protocol described in Methods in Cell Biol (Vol 129; page 153, 2015 issue). The fractions seem fine when probed for gamma-tubulin and histones, but I find almost all the nup proteins appearing in the cytosolic fraction that I further fractionate to enrich centrosomes.

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