Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

During transformation of Agrobacteriumu with plasmids ,PCR Positive colonies are not POSITIVE after restreaking.. Why?

Initially we are getting positive amplification .The positive colonies are selected and restreaked.The PCR is not showing positive amplification.

18 December 2020 9,802 3 View

How to calculate trapping parameter of the deconvolution peaks?

28 July 2020 2,413 2 View

Have any professors ever done research about the expression of fusion proteins in Drosophila S2 cells?

1. How to scale up the expression of fusion proteins? 2. How to improve the purity of fusion protein to 90%? I have tried affinity chromatography, but the purity by SDS-PAGE was not satisfied....

04 November 2019 8,479 6 View

How to calculate the active area of CNTs modified glassy carbon electrode?

"The active area of so porous electrode as the authors used could not be correctly evaluated using the Randles-SEVCIK formula. Just because the depletion layers of active places may overlap." How...

28 October 2019 1,782 3 View

Future of bioinformatic analysis in plant science ?

please share your idea regarding the future of bioinformatic analysis in plant science? recommend the preferred PC and MAC programs for the beginner ,and for the advanced users? as well as any...

24 October 2019 8,814 4 View

Raman cross section?

Can anyone help me to calculate Raman cross section in RE doped glasses?

12 December 2018 6,330 1 View

Which is the best kit/enzymatic method Efficient for the isolation of cells from the mouse or human tumors?

I want to isolate the cells from the tumors (mouse model and human). I read that Collagenase I is harsher than Collagenase IV. Can anyone please suggest if there is any commercial kit with the...

08 August 2018 4,961 2 View

Hi Everyone, can anyone tell how to get growth kinetics of bacteria with the help of Hoechst stain??

Hoechst 33258 stain

15 May 2018 3,128 3 View

Can any one give Jcpds fileof ZnSe and CdZnSe?

i have obtained an additional peak in XRD at 31.7 degree. so i want to get the idea about this peak few articles mentioned it but not showing angle values.

29 November 2017 3,772 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

What is the error of the Txy diagram?

Hi, I know the attached Txy diagram has an error but can't figure it out. Could you please look at the diagrams and explain it to me?

23 February 2021 4,239 1 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View