I would like to use FAM [5(6)-carboxyfluorescein] (ex 493/emission 517) fluorochrome in a Immunofluorescence protocol and I need to fix the staining. I can't find any fixation protocol convenient for this fluorochrome.
Thank you in advance.
Jason A Kilgore
Hi. In order to be fixable with aldehydes (such as formaldehyde or formalin) by crosslinking to proteins, the compound would have to have at least one primary amine on it. This reagent is not functionalized with a primary amine, and thus is not fixable.
For a fixable alternative, it would depend upon the usage you are wanting. I recommend you email us at Thermo Fisher Scientific Technical Support to get a recommendation, with details on your intended usage. You can write us at [email protected].
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