Hello Lina María García Forero,

If your experimental goal is to detect apoptosis and/or necrosis in kenyon cells of honeybee, I would suggest you to carry out the experiments as given below:

Take out the kenyon cells and fix them in 4% PFA on the slide and then carry out ICC using apoptosis markers to see the extent of apoptosis in the cell of your interest.

Once you find that your experimental protocol induces apoptosis/necrosis then try to harvest the cells (>10000 kenyon cells) and then conduct the flow cytometry using apoptosis/necrosis markers antiobody tagged to FITC/TRITC etc.

Apoptosis and necrosis occur during cell death in response to cytotoxic conditions. Annexin V-FITC binds to phosphatidylserine which is translocated from the inner to outer plasma membrane during early apoptosis. Propidium Iodide is a cell impermeable nuclear dye which is excluded by viable and early apoptotic cells. However, it is taken up by necrotic or late apoptotic cells resulting in red fluorescence.

Best