Can authors names in a publish paper influence the visibility of that particular article?

Can the position, grade, number of publication of scientists that authored a paper influence the visibility (acceptance, read, recommendation, citation,....) of an article during the submitting...

14 July 2020 3,011 8 View

What is the more adapted protocol for rat testes using NBF solution?

Hi all, I have found loads of paper to fix rat testes but plenty of protocol variations. I have started to fix some testes in NBF 10% for 24h but it doesn't look enough fixed. Do someone...

07 July 2019 4,721 1 View

Is possible to remove a co-author who only participated in the reasoning of a paper but who did not write or analyze anaything?

this is an ethical question. We have a conflict with a co-author who stopped its contributions, he only participated at the beginning of the project. I did all data analysis, article writing and...

04 March 2019 3,207 6 View

Extracting high frequency component from signal by using pywt in python?

I want to extract the higher frequency component from a signal composed of different frequency components by using the pywt package in python. How do I do this correctly? In order to extract the...

22 January 2019 4,940 1 View

Does anyone know if there is a method for the complex CRISPR/dCas9 to become fluorescent only when it binds to the gDNA?

Hi, I saw that it is possible to target and image a single locus on the genomic DNA with a cocktail of CRISPR/dCas9 following the CasFISH method. However, the signal is quite diffuse and I want to...

15 June 2018 1,583 2 View

Which company manufactures accurate temperature control enclosure?

I am looking for manufacturers that could produce customized temperature controlled enclosure ranging from +15 degC to +25 degC, with a precision lower than +/- 0.05 degC. This would fit on a...

18 March 2018 3,416 3 View

What are the conditions that you use in order to do immunostaining in differentiated 3T3-L1 cells?

Hello,I would like to do immunostainings on differentiated 3T3-L1 cells (adipocytes). We tried most of the "classic" protocols, fixation with either 4% PFA or cold methanol/acetone, and a lot of...

17 January 2017 8,549 3 View

How can I separate mouse brain vessels from parenchymal cells and assess purity of the fractions using immunoblotting (western blot/dot blot)?

Hi everyone, I am working on brain inflammation and I try to separate the mouse brain vessels from the parenchyma after the removal of the olfactory bulbs and the meninges. Currently I proceed to...

14 December 2016 4,163 2 View

I have trouble with my human NPC. Sometimes they detach from the coating (Po+Lam) and form neurospheres. any idea to avoid this?

randomly NPC can detach from the coating and form neurosphere. The coating is fresh and always the same, the medium also.

22 August 2016 299 2 View

How to obtain CI for beta coefficient in SPSS 22 after a multiple linear regression ?

In SPSS 22.0, i obtain value for b and for beta (standardised coefficient) for each variable i use in my multiple linear regression modelization. However, confindence intervall are given for B...

29 March 2016 2,208 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View