U can refer to following for information on compatible reagents: http://www.bio-rad.com/webroot/web/pdf/lsr/literature/4110065A.pdf. If you protein concentration is very high, then you can dilute the sample to reduce the effect of the reagent/component which you are doubting.

Tanks.i 'll try with dilution as well

What is your mixture? Does it contain a lot of salt?

On which buffer are your proteins? Could it be you have a non physiologic pH?

microtiter plate bradford should normally be controlled for interfering compounds more stringently than normal bradford. If you cannot omit the interference (e.g., detergent, reducing agent etc), try alternatively bicinchonic acid assay for microplate readers (supplied by Pierce). Colour is more stable although it needs longer incubation time to develop.

I'm in favor of the simpler Lowry modified by Peterson assay... quick, easy, reproducible and less interferents.

Thanks for ur helps:)