12 Questions 182 Answers 0 Followers
Questions related from Nicolas Luigi Pascal Casadei
I found once on the web a website which allow scientists to share the review they made from scientific articles, but cannot remember the address.
03 March 2015 7,095 8 View
I try to read enzymatic activity in tissue homogenates using fluorescence emission in a microplate reader. I hypothesize that a specific treatment can reduce amount of enzyme in tissue and/or...
01 January 2015 6,859 11 View
In immunohistochemistry, many protocols recommend to block the tissue to analyze prior the first antibody incubation with the serum of the animal in which the secondary antibody is raised...
07 July 2014 7,949 21 View
I noticed that some CO2 suppliers provide different range of puritz (from 99.0% to 99.99%). I was wondering how much CO2 quality may impair or enhance reproducibility of cell culture...
07 July 2014 8,567 9 View
We are collecting blood from mice sacrificed by cervical dislocation by removing an eye and let blood drop by one eye. This method provide us around 300 to 500 µl of blood per animal. We let the...
10 October 2013 7,097 8 View
Does anybody have a good reference on the number of cells (including neurons, glial cells...) present in 1µg of mouse brain tissue? More precisely on pre frontal cortex, hypocampus, or striatum...
08 August 2013 6,204 8 View
I was wondering what would be the effect of too high a concentration of APS in an acrylamid gel. I am also curious to know how people manage their stock of APS (we dilute APS with water at 10%...
01 January 2013 7,818 13 View
Are antibodies already HRP-conjugated more sensitive than the two steps "antibody + washing + anti species HRP-conjugated"? If yes, kind you suggest any kit to do it?
12 December 2012 6,497 11 View
I would like to buy plasmids from mouse. My aim is to clone a protein of interest inserting a promoter and a tag (we already choose them and have them in the lab). We would then like to transfect...
10 October 2012 6,741 47 View
Sorry for this relatively simple question which need a long explanation In a experiment, --> to illustrate: I am counting the number of step that mice need to walk trough a 10 cm long beam I...
09 September 2012 8,229 11 View
As previously posted on the forum, we are using an illuminated glass plate to measure gait disturbance in mice. As you can imagine, speed is directly positively correlated to a high number of...
09 September 2012 3,220 10 View
We are transfecting HEK cells using a vector expressing a protein normally located in the nucleus of cells. By mutating the nuclear localization signal of the protein of interest, we would like to...
09 September 2012 2,503 14 View
