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Questions related from George Komis
I am looking for plasmid or bacteria expressing 6xHis-tagged cutinase from Erysiphe graminis for purification. Thanks a lot in advance
10 October 2017 8,319 0 View
Anyone has experience with a truly permeable DNA stain that will not be adsorbed in plant cell walls? We work on Arabidopsis roots and we tested dyes that are membrane permeable in animal cells...
11 November 2015 1,987 7 View
I need fluorophore conjugated anti-rat and anti-mouse immunoglobulins that do not crossreact. Anyone with a reliable source of cross-adsorbed IgGs of this kind?
04 April 2015 5,224 4 View
I am looking for efficient kits for Co-IP using mouse and rabbit IgGs. Effectively co-IP output should be minimally contaminated with IgG heavy/light chains. Any idea?
09 September 2014 4,782 10 View
I need a link to an online tool where I can upload small peptide sequences or FASTA format proteins that do not necessarily have a PDB accession. Any idea?
07 July 2014 7,042 2 View
Homo- or heterobifunctional crosslinkers were sparsely reported in the literature as putative fixatives for immunolocalization microscopic studies. Does anybody have information on which...
04 April 2014 1,661 1 View
Does anyone have experience with high pressure fixation regimes for plant tissues with different vacuolation degrees. Primarily I would like to apply HPF to Arabidopsis roots, but I would greatly...
03 March 2013 4,798 1 View
Does anyone have any ideas about high refractive index mounting media for either fixed, immunolabeled samples or for living, GFP-fusion expressing cells, to avoid spherical aberrations with high...
02 February 2013 8,270 2 View
Can somebody attach a step by step and thorough protocol for aqueous two phase partitioning of Arabidopsis root plasma membranes? I am not experienced and I need an easy to follow approach.
10 October 2012 5,037 4 View
For example formaldehyde, glutaraldehyde or acrolein? I am particularly interested to understand whether soluble proteins may be largely relocated during chemical fixation.
09 September 2012 9,152 26 View
Does anybody have experience with GFP fluorescence preservation following aldehyde or other kind of fixation? In our hands formaldehyde fixation (from freshly hydrolyzed 4% paraformaldehyde),...
09 September 2012 7,548 88 View
I want to ask whether growth conditions of Arabidopsis seedlings could affect the efficiency of transgene expression driven by the 35S promoter of cauliflower mosaic virus. I am having problems...
09 September 2012 9,930 26 View
Anybody has an idea whether mpk4 mpk6 double knock out mutants were ever studied?
09 September 2012 456 0 View
Does anyone have experience with hammarsten grade casein blocking for pvdf membranes? Is it a better substitute for milk? Is it more suitable for phosphopeptide detection?
07 July 2012 4,119 4 View
Can anybody provide advice on preservatives for blocking solutions or antibody diluents other than sodium azide? I normally use HRP based chemiluminescence detection and I should avoid sodium azide.
07 July 2012 7,713 11 View
Heavy background on pvdf membranes blocked with 5% skimmed milk and probed with affinity purified rabbit pAbs I am probing plant MAPK species using commercially available antibodies from Sigma....
06 June 2012 3,533 7 View
