I am using a Biorad real time pcr device which is quiet old and cyber green method. I have made different dilution twice (once dilution factor 2 and second time 5) four series from first to the last one they have been decreased.but the problem is that my third dilution's curve rose sooner than the forth(dilution factor 2) and I reaped it with dilution factor 5 now the second and first have a same problem. do u think my primer has a problem.