This is my protocol for sampling serum from mice (or rat):
1. Take a blood from vena cava with syringe.
2. Put the blood in 1.5 mL E-tube, carefully.
3. 30 min in RT for clotting.
4. And centrifuge 10000rpm, 10 min
Does anyone have a better protocol for this?
yes~ you are right ! You also can tune up the centrifuge in 4 degree than 10000rmp, 10 min~
Hi, the rpm depends on the size of the centrifuge. We've used 2500g, 15 min and 10 degrees. For maximum yield, you can suck of everything that has not clotted and centrifuge again. Then take off only serum.
Dear all,
I am planning to do a immune response study in pigs and I have a doubt that I hope someone can clarify me:
I need to get blood from the pigs and then extract serum and PBMCs (Peripheral Blood Monocytes cells) from the blood. Does anyone know whether I can freeze the blood and make up the serum and extract the PBMCs after freezing the blood?
If someone can clarify my doubt I'd be really grateful.
Thanks in advance.
Iker
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