16 Questions 17 Answers 0 Followers
Questions related from So-Jin Kim
Hello, Sometimes, I have to protect the samples/plates from light such as ELISA, immunofluorescence, etc. I'm usually perform the procedures under the normal room-light, and cover the...
07 July 2018 7,297 5 View
Hi all, I have primers for ChiP analysis in my lab. Before I start the experiments, I'd like to check this is really target for p16INK4A gene (CDKN2A). Q1. How can I check the target promoter...
05 May 2018 4,504 7 View
Hi all ! I've used 30:1 acrylamide/bisacrylamide solution (Bio-Rad) for making WB gel. Once I run the gel, the line in separating gel suddenly showed up (please see the attached figure). It really...
01 January 2016 6,171 9 View
Hello all :) I am choosing the primary antibody for WB analysis. During look around, I found there is different predicted band size for this protein. In same company, two primary antibodies are on...
04 April 2015 8,896 6 View
Hello Recently, I received the comments of referee that I should detect the TEM to confirm the necroptosis. However, I wonder there is a tool for this. Is there any histological and/or TEM...
04 April 2015 6,314 4 View
I plan to assay total cholesterol with "cholesterol assay kit". (Cayman, Cat.# 10007640) Instruments said to dilute the assay buffer 10X into 1X with HPLC-grade water. So, first I want to buy it,...
03 March 2013 3,361 28 View
I'm interested in the relationship between HDAC (histone deacetylase) inhibitor (HDACi) and sepsis. With my recent experiment, I got different patterns between mRNA and ELISA as a result of some...
12 December 2012 1,006 3 View
Recently I did an immunoprecipitation to determine the affinity between two proteins and I got a good result from that. But, I wonder is there any method to determine affinity between three...
12 December 2012 3,401 14 View
I used Totallab TL120 software for density analysis of WB bands. But sometimes I am faced with the problem of "inverted images". The software automatically converted band images to software...
12 December 2012 657 4 View
I want to know Erk activity in my animal model - sepsis. I isolated it from liver, homogenized it, separated it by SDS-PAGE, and detected it, but I just found one band for Erk. Procedure was the...
12 December 2012 6,564 17 View
I have new compound and I will experiment whether its effect on sepsis model in mouse. First I have to find out its solubility, but there are some problem with this. I would like to predict its...
09 September 2012 8,775 8 View
I am processing immunoprecipitation with RAW 264.7 cell lysis. After protein assay, it was diluted with DW not IP buffer (IP buffer = RIPA buffer + PBS + phosphatase inhibitor + protease...
08 August 2012 5,113 1 View
I try to find efficacy of drug in septic mouse model. And I have problem with drug's solubility because it is insoluble in water (saline/PBS/etc.) So, I have to use DMSO as its solvent and I find...
05 May 2012 5,918 10 View
This is my protocol for sampling serum from mice (or rat): 1. Take a blood from vena cava with syringe. 2. Put the blood in 1.5 mL E-tube, carefully. 3. 30 min in RT for clotting. 4. And...
05 May 2012 4,620 5 View
I want to do histology score for organ damage (heart, lungs, kidneys, and liver). So I will stain with H&E and then score that. But, there is problems how can I deal with tissue? Others say...
05 May 2012 9,677 18 View
It is the first time I order primer for specific DNA. I want to make a primer (reverse PCR) for mouse MKP-1 DNA and I don't know how can I make it! Can I have any information needed to make...
05 May 2012 8,853 5 View
