Please share your experience and the Suggestion for Whole Exome data analysis..(for case-control study)

07 August 2017 5,311 1 View

Which one is better for Primer dilution Distilled water or TE buffer ( GC rich - 63% )

I usually use distilled water for primer dilution, i never got problem but one of GC rich (63%) primer getting degraded often. which one can be better for GC rich primer dilution TE or water if TE...

06 July 2017 2,915 26 View

Does min6 cells change their morphology when performing GSIR using KRBH?

08 September 2015 2,696 0 View

What is the difference between static and perifusion insulin assays?

06 July 2015 3,367 0 View

Can someone provide a protocol for DNase I treatment after RNA extraction?

01 February 2015 9,792 4 View

I am checking DNA/RNA conc. & purity in nano drop, for purity I am seeing a peak at 260/280 nm, what does it confer? Peaks are also coming at other nm.

When i check RNA purity the peak height at 260 or 280nm is less than the peak height at 230nm or other nm.

11 December 2012 1,823 10 View

If i will use a single primer in a PCR, templet is genomic DNA did i get a amplified product ? how ? when single primer is used ?

if i will run the gel of that PCR product did i will get any band ?

31 December 2011 9,121 15 View

Need a Phospholipid estimation kit

Based on colorimetric method or any other method

31 December 2011 5,717 0 View

I need a procedure for cholesterol extraction and estimation from biological samples ?

The problem is biological sample having bile pigments, it interfering while estimating the cholesterol..

31 December 2011 8,009 16 View

About ELISA reader .... what is the basic principle ???

ELISA reader at particular nm length absorbs only one color or more ?? EX : at 505 nm length it absorbs pink if i will put yellow color reaction sample its giving high value than pink color. why...

11 December 2011 7,643 2 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why does Reaction Wood acts opposite in gymnosperm and angiosperms?

I am wondering why the Gymnosperms have an opposite behaviour that Angiosperms in the Reaction Wood (RW). Are there some new findings regarding that? Studies say that causal agents can be the...

10 January 2021 8,744 4 View

What are the essential elementts of a Nature Neuroscience Paper?

What would land a neuroscience paper in Nature Neuroscience? What is the minimum a paper should have to pass the editorial scrutiny in Nature Neuroscience or the journals around the same cadre?...

01 January 2021 1,510 2 View

What is the role of fresh cow dung in sprouting in stem cutting propagation?

In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.

24 December 2020 4,068 6 View

Why the taste of foods(item) changes with the sources of fuel we used(bio fuel,wood,dried dung) reality or meth ?

As my experience and many consumer experience they feel the different taste of the same item served cooked in a different source, is it real or not, what is the reason behind this.

24 December 2020 6,476 3 View

What is the level of consciousness , communication and feeling in plants ?

Do you think the plant can communicate with each other, what is the level of feeling in plants?

22 December 2020 2,754 17 View