Please share your experience and the Suggestion for Whole Exome data analysis..(for case-control study)

07 August 2017 5,311 1 View

Which one is better for Primer dilution Distilled water or TE buffer ( GC rich - 63% )

I usually use distilled water for primer dilution, i never got problem but one of GC rich (63%) primer getting degraded often. which one can be better for GC rich primer dilution TE or water if TE...

06 July 2017 2,915 26 View

Does min6 cells change their morphology when performing GSIR using KRBH?

08 September 2015 2,696 0 View

What is the difference between static and perifusion insulin assays?

06 July 2015 3,367 0 View

Can someone provide a protocol for DNase I treatment after RNA extraction?

01 February 2015 9,792 4 View

I am checking DNA/RNA conc. & purity in nano drop, for purity I am seeing a peak at 260/280 nm, what does it confer? Peaks are also coming at other nm.

When i check RNA purity the peak height at 260 or 280nm is less than the peak height at 230nm or other nm.

11 December 2012 1,823 10 View

Bile salt estimation?

Can anyone recommend a protocol for quantitative estimation of bile salts in bile sample?

02 March 2012 7,653 2 View

If i will use a single primer in a PCR, templet is genomic DNA did i get a amplified product ? how ? when single primer is used ?

if i will run the gel of that PCR product did i will get any band ?

31 December 2011 9,121 15 View

I need a procedure for cholesterol extraction and estimation from biological samples ?

The problem is biological sample having bile pigments, it interfering while estimating the cholesterol..

31 December 2011 8,009 16 View

About ELISA reader .... what is the basic principle ???

ELISA reader at particular nm length absorbs only one color or more ?? EX : at 505 nm length it absorbs pink if i will put yellow color reaction sample its giving high value than pink color. why...

11 December 2011 7,643 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View