Please share your experience and the Suggestion for Whole Exome data analysis..(for case-control study)

07 August 2017 5,311 1 View

Which one is better for Primer dilution Distilled water or TE buffer ( GC rich - 63% )

I usually use distilled water for primer dilution, i never got problem but one of GC rich (63%) primer getting degraded often. which one can be better for GC rich primer dilution TE or water if TE...

06 July 2017 2,915 26 View

What is the difference between static and perifusion insulin assays?

06 July 2015 3,367 0 View

Can someone provide a protocol for DNase I treatment after RNA extraction?

01 February 2015 9,792 4 View

I am checking DNA/RNA conc. & purity in nano drop, for purity I am seeing a peak at 260/280 nm, what does it confer? Peaks are also coming at other nm.

When i check RNA purity the peak height at 260 or 280nm is less than the peak height at 230nm or other nm.

11 December 2012 1,823 10 View

Bile salt estimation?

Can anyone recommend a protocol for quantitative estimation of bile salts in bile sample?

02 March 2012 7,653 2 View

If i will use a single primer in a PCR, templet is genomic DNA did i get a amplified product ? how ? when single primer is used ?

if i will run the gel of that PCR product did i will get any band ?

31 December 2011 9,121 15 View

Need a Phospholipid estimation kit

Based on colorimetric method or any other method

31 December 2011 5,717 0 View

I need a procedure for cholesterol extraction and estimation from biological samples ?

The problem is biological sample having bile pigments, it interfering while estimating the cholesterol..

31 December 2011 8,009 16 View

About ELISA reader .... what is the basic principle ???

ELISA reader at particular nm length absorbs only one color or more ?? EX : at 505 nm length it absorbs pink if i will put yellow color reaction sample its giving high value than pink color. why...

11 December 2011 7,643 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View