hi
i found a change in the morphology of min6 cells while doing GSIR. some cells even got lifted from the plate. is this common while performing the GSIR using Min6 cells?
i did whole exmoe sequencing using (NGS) ion Proton system .. i have the patients and control data... need suggestion and the pipelines filters for data analysis how to avoid and know the false...
07 August 2017 5,425 1 View
I usually use distilled water for primer dilution, i never got problem but one of GC rich (63%) primer getting degraded often. which one can be better for GC rich primer dilution TE or water if TE...
06 July 2017 3,043 26 View
Hi I generally do GSIR by static method. Recently i found Perifusion method to study insulin release. So what is the difference between these two methods and which is more reliable? Thanks
06 July 2015 3,475 0 View
I have some frozen RNA samples in -80 and I want to do cDNA synthesis fallows qRT-PCR. I am using DNase I from genei i want use EDTA for deactivation of Dnase I after treatment with RNA thank you..
01 February 2015 9,911 4 View
When i check RNA purity the peak height at 260 or 280nm is less than the peak height at 230nm or other nm.
11 December 2012 1,976 10 View
Can anyone recommend a protocol for quantitative estimation of bile salts in bile sample?
02 March 2012 7,757 2 View
if i will run the gel of that PCR product did i will get any band ?
31 December 2011 9,216 15 View
Based on colorimetric method or any other method
31 December 2011 5,822 0 View
The problem is biological sample having bile pigments, it interfering while estimating the cholesterol..
31 December 2011 8,117 16 View
ELISA reader at particular nm length absorbs only one color or more ?? EX : at 505 nm length it absorbs pink if i will put yellow color reaction sample its giving high value than pink color. why...
11 December 2011 7,749 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View