Govardhan Bale

12 Questions 20 Answers 0 Followers

Questions related from Govardhan Bale

Please share your experience and the Suggestion for Whole Exome data analysis..(for case-control study)

i did whole exmoe sequencing using (NGS) ion Proton system .. i have the patients and control data... need suggestion and the pipelines filters for data analysis how to avoid and know the false...

08 August 2017 5,381 1 View

Which one is better for Primer dilution Distilled water or TE buffer ( GC rich - 63% )

I usually use distilled water for primer dilution, i never got problem but one of GC rich (63%) primer getting degraded often. which one can be better for GC rich primer dilution TE or water if TE...

07 July 2017 2,990 26 View

Does min6 cells change their morphology when performing GSIR using KRBH?

hi i found a change in the morphology of min6 cells while doing GSIR. some cells even got lifted from the plate. is this common while performing the GSIR using Min6 cells?

09 September 2015 2,758 0 View

What is the difference between static and perifusion insulin assays?

Hi I generally do GSIR by static method. Recently i found Perifusion method to study insulin release. So what is the difference between these two methods and which is more reliable? Thanks

07 July 2015 3,430 0 View

Can someone provide a protocol for DNase I treatment after RNA extraction?

I have some frozen RNA samples in -80 and I want to do cDNA synthesis fallows qRT-PCR. I am using DNase I from genei i want use EDTA for deactivation of Dnase I after treatment with RNA thank you..

02 February 2015 9,868 4 View

I am checking DNA/RNA conc. & purity in nano drop, for purity I am seeing a peak at 260/280 nm, what does it confer? Peaks are also coming at other nm.

When i check RNA purity the peak height at 260 or 280nm is less than the peak height at 230nm or other nm.

12 December 2012 1,912 10 View

Bile salt estimation?

Can anyone recommend a protocol for quantitative estimation of bile salts in bile sample?

03 March 2012 7,708 2 View

If i will use a single primer in a PCR, templet is genomic DNA did i get a amplified product ? how ? when single primer is used ?

if i will run the gel of that PCR product did i will get any band ?

01 January 2012 9,175 15 View

I need a procedure for cholesterol extraction and estimation from biological samples ?

The problem is biological sample having bile pigments, it interfering while estimating the cholesterol..

01 January 2012 8,065 16 View

Need a Phospholipid estimation kit

Based on colorimetric method or any other method

01 January 2012 5,777 0 View

About ELISA reader .... what is the basic principle ???

ELISA reader at particular nm length absorbs only one color or more ?? EX : at 505 nm length it absorbs pink if i will put yellow color reaction sample its giving high value than pink color. why...

12 December 2011 7,709 2 View

I want to estimate bile salts and bile phospholipids in bile sample i need a conventional method or a kit method please help me out

If i will use calorimetric/sepc method bile pigments also give some color to reaction

10 October 2011 6,942 0 View