Hi everyone,
I have found a few articles that give recipes for making cell culture media specifically for human neutrophils. I would like to do experiments where I would procure and magnetically isolate human neutrophils and then observe their behavior the same day or one day later at the latest. Given this, please share your thoughts. Here are a few other q's: Is RPMI or IMDM a better base media? Does it even make a difference given the short timeframe? Is 10% FBS or 20% FBS better? Is L-glut necessary?
Thanks so much for your input!
Hello Chad D Markert
You cannot maintain human neutrophils for more than 24 hrs. because after 24 hrs. apoptotic cells are observed (more than 50%). RPMI 1640 will be a better basal medium with 10% FBS. No need to add L-glutamine.
Best.
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