I need to isolate and purify the T.annulata Sporozoites, for using as an Antigen.
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...
06 August 2024 9,986 2 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View
Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...
01 August 2024 572 0 View
HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...
31 July 2024 5,309 7 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out? For example consider a process for certain product manufacture where a new process flow...
31 July 2024 781 1 View
Hello! I planned to do an multiplex RNAscope fluorescent assay for cultured cells in a 96 well plate format according to the protocol provided by ACD:...
22 July 2024 3,334 0 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...
06 July 2024 4,521 2 View
I would like to find a short course focused on the development of monoclonal antibodies covering the entire process, from the selection of antigen and carrier, to the purification of the final...
27 June 2024 5,618 1 View
I am trying to quantify the release of Nitric oxide to test the Immunogenicity of my antigen on U937 cells (Monocytes). Based on the literature, I am supposed to convert 1x105 cells/ml to...
27 June 2024 3,131 2 View
In the molecular dynamics simulation analysis part, how to calculate molecular surface area (MolSA) and polar surface area (PSA) in gromacs ?
14 June 2024 1,469 1 View
How can I use EDSV fiber protein antigen for hemagglutination test?
05 June 2024 1,459 0 View
Hello everyone, hope you are doing well. I have FFPE brain arteriovenous malformation (AVM) tissues I have been trying to stain with cleaved-caspase-3. I have used the Asp175 (D3E9) CC3 9579 from...
11 May 2024 1,695 2 View
I have two antibodies that are staining well in IHC but not in IF for tissue sections, when using the same concentration and antigen retrieval method for both. I also know the secondary works well.
08 May 2024 3,890 4 View
I want to ask if antigen retrieval process works for formalin fixed tissues of more than 7 days or even 2-3 months ? Or it should be done for tissues that are fixed for max upto 24 hours ?
05 May 2024 2,413 2 View