Eugene Cheung

4 Questions 7 Answers 0 Followers

Questions related from Eugene Cheung

Are there any options for high-throughput parallel genomic and transcriptomic single cell sequencing?

I am interested in parallel genomic and transcriptomic sequencing at the single cell level but with the high throughput capacity of a system like 10X. I understand that this is doable at a...

04 March 2022 9,310 3 View

Best kit for generation of single nuclei suspension from mouse brain tissue?

Hello! I am looking to isolate cell nuclei from mouse brains (hypothalamus specifically) and have been considering several kits. There are two from Sigma - the Nuclei EZ Prep and Nuclei Pure...

16 June 2021 655 1 View

Brain removal technique from ~18cm fish?

I am trying to remove brains from medium-sized cichlids ranging in size from 16-18cm total length. In previous work with substantially smaller fish (TL: 2-3cm), I was successful in cutting the...

19 February 2018 1,340 2 View

How do I lyophilize my recombinant protein?

I have been working on purifying a 16kDa His-tagged protein for the past year (literal year -- began this project on May 6, 2016). I finally refined my protocol to the point that I was getting...

28 April 2017 8,018 11 View