Could anyone help me find the best composition for cell lysis solution for manual DNA extraction using blood as the source? I want to prepare the solution in house. Does anyone have experience preparing it?
You can use different detergent like CHAPS, SDS, Tween-80 or NP-40. But its is very important to separate the protein from the DNA in order to get pure DNA which can be further used for the PCR, Blotting or various purposes. You can use Proteinase K for protein separation.