I am trying to run WB to detect full-length AR and AR-V7 in sc xenograft tumor tissue generated from 22Rv1 human prostate cancer cells (implanted in mice). I've been using Santa Cruz AR (441) antibodies, but the results have been fairly poor. There is a lot of background and non-specific binding, and the desired bands are not very sharp. Band intensities of the same samples vary considerably from one experiment to the next, in spite of a fairly uniform and consistent loading control (B-actin). Would anyone know of a good quality AR antibody for this application? Or has anyone used AR (441) successfully in xenograft protein samples? Incidentally, I have used this same antibody for IHC in these tissues, and I have had good results.
I think, we used this clone from Cell Signaling for a similar study (Article Anti‐cancer function of microRNA ‐30e is mediated by negativ...
) that included staining xenografts and Western blotting (supplemental figures S2 and S3):https://www.cellsignal.com/products/primary-antibodies/androgen-receptor-d6f11-xp-rabbit-mab/5153?site-search-type=Products&N=4294956287&Ntt=ar&Nrpp=30&No=0&fromPage=plp
Thank you so much for replying, Dr. Andl and Dr. Iborra. Most appreciated!
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