Gisella Campanelli

4 Questions 6 Answers 0 Followers

Questions related from Gisella Campanelli

Best AR antibodies for human prostate tumor xenograft in mice?

I am trying to run WB to detect full-length AR and AR-V7 in sc xenograft tumor tissue generated from 22Rv1 human prostate cancer cells (implanted in mice). I've been using Santa Cruz AR (441)...

22 October 2022 231 2 View

How to prepare Enzalutamide for i.p. injection?

In an upcoming in vivo experiment, we will be administering enzalutamide by i.p. injection in nude mice. We plan to dissolve Enzalutamide in DMSO, and then dilute this solution using normal...

17 May 2022 8,873 3 View

G418 concentration required for selection of luciferase-tagged 22Rv1 cells?

I am using G418 to select for stably transduced 22Rv1-luciferase expressing cells. Would anyone know of the minimal effective concentration of G418 required to kill 22Rv1 parental cells? I am...

11 November 2021 8,470 3 View

Would anyone have advice regarding the maintanence and handling of 22Rv1 cells?

I am having some difficulties with my 22Rv1 cells. I am finding that a lot of debris accumulates in my plates. Washing with DPBS only partially helps. Also, dissociation from the plate can take...

08 June 2021 3,378 9 View