Besides T7 RNA Polymerase, is there any other protein that binds the T7 promoter?

More Shai Zilberzwige - Tal's questions See All

Where can I get "whole organism" mRNA expression levels (of many organisms)?

Hey, I need to get the mRNA expression profile of multiple organisms, and I do not need it to be specific for a certain tissue or status, but rather the average values per organism. For example,...

09 December 2020 3,620 3 View

Best way transfecting two sgRNA's on two different CRISPR/CAS9 plasmids?

I'm trying to knock out a gene in mESCs. I have my guides already cloned each into a CRISPR/CAS9 plasmid also carrying GFP. My guides didn't work after transfecting them individually, so I wish to...

03 November 2020 6,468 3 View

Is the ICBM-DTI-81 Atlas acceptable for use?

I am interested in using this DTI atlas ( http://www.bmap.ucla.edu/portfolio/atlases/ICBM_DTI-81_Atlas/ ) but came across a publication suggesting it is not oriented properly (...

19 April 2020 6,770 4 View

Which sensory-motor learning task and problem solving task are well-researched and easy to administer (do not require special & expensive equipment)?

We are planning a study in which we will explore the effects on the brain of a sensory-motor intervention which simulates the process of developmental sensory-motor learning. We plan to explore...

27 December 2018 6,935 1 View

Which resources could help residents develop a successful grassroots participatory process ?

In my article "When knowledge is power: grassroots participatory initiative as a process of resource development" I claim that residents are initiating informal grassroots participatory...

25 December 2017 1,657 12 View

Is slashing and mulching of Asparagus leaves at the end of the growing season more efficient than allowing nutrient translocation to take place?

In Australia, commercial asparagus farms cut back vegetative growth and mulch it into the soil at the end of the growing season in order to return the nutrients in these plant parts to the soil....

19 November 2017 988 3 View

Can any one give me a protocol for beta gal ONPG assay for AML12 transfected cells?

I have a protocol which we used a few years ago with 3T3 cells. With the AML12 cells I don't get any color. Any suggestions? Thank you.

30 October 2017 1,749 1 View

How can I save multiple output files from Kubios at once?

I have 300+ participants to analyze in Kubios for heart rate variability. Currently, the only option I know of, is to save each data file one by one, which is very time consuming. Is there a way...

24 September 2017 8,707 1 View

How to merge files and define new values on SPSS?

Well, I'm facing some troubles with SPSS. I'm trying to merge 2 files and I will try to explain the problem with a simple example: Let's say I have names of patients in the "master" file, while on...

14 September 2017 5,619 3 View

Which statistical model(s) is this paper using?

Differential tolerance to metals among populations of the introduced bryozoan Bugula neritina (Piola and Johnston 2006) I have been asked to review this paper and comment on the statistical models...

16 August 2017 9,131 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Marko Dolinar

I wouldn't believe there is anything except T7 polymerase in specific E. coli strains (I assume you work with bacteria) that could bind to T7 promoter sensu stricto. However, in some vectors, T7 promoter is combined with an operator region (lac mostly) without making this clear on the vector map. You should be able to find this out by checking the sequence downstream of the promoter.

Shai Zilberzwige - Tal

Thanks