Dear J. Cl

OCTET (BLI approach) is certanilly an possible powerfull method, fast and reilable.

https://www.sartorius.com/download/552288/fast-quantitation-of-proteins-and-antibodies-using-octet-n1-system-application-note-en-sartorius-data.pdf

of course it is quite expensive apparatus that can be used also for other purpose (eg. affinity determination of purified nabs and self aggregation propensity evaluation of the purified mabs)

the size and throughput of the instrument depends from the number of channel (from 2 to 96). i had the opportunity to work with the 8 channel and i think that at least the 4 channel version is necessary.

to my knowledge there are also other immonoassay tecnologies as the Gyrolab platform

https://www.gyrosproteintechnologies.com/immunoassays/product-titer-quantification-applications#:~:text=Gyrolab%20human%20IgG%20titer%20Kits,dilutions%20and%20repeats%20for%20analysis.

that can do the same work but also in this case are quite expensive methods.

SDS-page and UV quantification of protA/protG purified mab in small scale (eg purification of 2ml surnatant with 100ul of resin by gravity or vacum manifold) are the cherape alternativelly but are of course applicable only in a limited number or samples in parallel 10-20 not 100.

Capillary elettrophoresis may be an more quantitativa alternative to SDS-page, but since the instument is quite expensive, i prefer the OCTET approach which may allow to you to perform many other things.

good luck

Manuele