Hi.
I am using a BCA protein assay. Using 200uL of Working Reagent and 10uL of the protein isolate. I am reading the results at 562nm absorbance (see image). After that point, should I dive the values by 20? (10:200, 1:20 dilution as the kit instructs) then calculate the desired amount of protein (if need 100ug, 100/protein) Or, should I directly divide the value I got from the reads without dividing to 20, and find the amount I need to load onto the gel?
Hi,
It is dependent on the dilution rate that you used during preparation of samples. For example, if I have x1 sample and take 1 microliter and add 9 microliter of the diluent. Then you have to multiply your results with 10. So, the dilution rate here is not affected by the volume of your reagents mix.
Best..
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