Basic question - how to make a cell dissociation buffer?

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Sam Siljee

I have my answer - the calculator I was using wasn't updating properly when I changed between units of mass.

Samy Azer

Thank you, Sam Siljee for your question. It depends on the type of cells you are going to isolate. I hope these methods help

https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/cell-isolation

Thank you Samy Azer,

Another useful resource I've found is from the Worthington website, where they list references for dissociation protocols by tissue type:

http://www.worthington-biochem.com/tissuedissociation/tissuetypes.html