Bacteroides fragilis 3_1_12 bft positive?

More Katie Lennard's questions See All

Acquity H-Class UPLC troubleshooting help?

I apologize in advance for the length of this post but there is a lot of information. Our lab has an Acquity TQD coupled with an H-Class UPLC (Column manager-CM, sample manager-SM, and quaternary...

03 June 2024 6,448 3 View

Is it possible to detect a compound binding to oligo in an EMSA, mobility shift assay?

If so do you have to crosslink the compound to the oligo? If you have done this kind of experiment I would really appreciate any guidance/protocols. Thanks, Katie

21 May 2024 318 1 View

Hi all, i am having some trouble choosing the right statistical test for my disk diffusion assay results, can anyone help?

i have recently done a disk diffusion assay where by i measure the zone of inhibition of 5 antibiotics on 7 different pathogens, i want to establish if there is any significant difference between...

12 March 2024 1,250 5 View

What is the best fluorescent dye to add to sodium alginate?

I am mixing a sodium alginate gel to apply to microfluidic channels, I’d like to use a fluorescent dye to visualise where it has been deposited after application. It would have to be oleophobic...

25 February 2024 8,205 1 View

Quality Checks for Eddy-Corrected Diffusion Data?

I currently have some diffusion data for young children that we are trying to analyze. We are using FSL's eddy motion correction, but as we are looking at subcortical white matter pathways, we...

24 July 2023 1,951 2 View

Stability & storage of salivary alpha amylase?

I'm designing a new activity for an undergraduate biology course about enzyme activity. I'm running into a slight complication. I've ordered a lyophilized powdered version of human salivary...

12 July 2023 686 6 View

Is there a commercially available MAC glycopeptidolipid (GPL) core antigen?

I am looking for a commercially available GPL core antigen to use in a Luminex immunoassay. I am aware of the Capilia MAC Ab ELISA kit, but I am searching for just the antigen to couple to Luminex...

01 March 2023 9,433 0 View

How to analyze action sequences for consistency?

Has anyone analyzed intra- and inter-individual consistency in task order (i.e., repeatability)? I have repeated measures of multiple individuals and would like to see if a consistent action...

27 February 2023 337 3 View

What inferential statistical test should I use to compare the result?

I have asked my students to undertake differently worded questions on the same subject matter; Q1A & Q1B, Q2A & Q2B, Q3A &Q3B and so on, , I have then asked them to express agreement using a...

23 August 2022 7,724 8 View

Does fatty acid availibility affect glucose uptake?

Hi! I am measuring changes in glucose uptake in primary adult cardiomyocytes after addition of fatty acids. I am using a 1:100 dilution of CD lipids. Glucose uptake is measured by fluorescent...

14 July 2022 951 1 View

How does the human microbiome influence health and disease, including implications for personalized medicine and microbiome-targeted therapies?

Probiotics related.

22 July 2024 1,707 2 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

What role does the gut microbiome play in immune system modulation and disease susceptibility?

Only Probiotics Related

22 July 2024 6,454 0 View

How to ask to correct the Incorrect Claim regarding the writer of Article that I was involved in?

Dear sir/Madam, I am Parinaz Parsa the first writer of article "Optimisation of probiotic yoghurt production enriched with phytosterols" and understand that one person is mentioned as writer which...

14 July 2024 9,579 2 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

Can all postbiotics survive the gastric journey or is it again strain-specific or will depend the type of postbiotic?

General understanding is that postbiotic particularly those that are heat-treated are stable to heat and their activity remains stable during gastric journey. Wondering if this is true for all...

25 June 2024 3,133 3 View

Did you ASK AI (https://talkai.info/tr/chat/) the same question?

What food do intestinal bacteria like most?ChatGPTIntestinal bacteria love fibrous foods that are prebiotic. These foods include vegetables (such as broccoli, cabbage, and brussels sprouts),...

22 June 2024 4,567 0 View

Can increasing bacterial concentration in a formulation compensate for inhibition by lactobacillus strain,and if so ,is it right to do so?

I am conducting research on the antimicrobial synergism activity of lactobacillus strain with B.subtilis. in a recent experiment, we observed inhibition of B.subtilis growth with high...

01 June 2024 338 2 View

What is the function of CaCl2 in the lipolytic assay? what percentage concentration of CaCl2 should be added in the lipolytic assay?

Hello everyone. I need some information about the use of CaCl2 in lipolytic assays. I often see the addition of CaCl2 in lipolytic activity assays, especially in probiotic screening, but there is...

08 May 2024 7,480 2 View

Is it good to have antibiotic resistance property for probiotic?

To prevent intestinal disorders, probiotics can be rendered multidrug-resistant to survive in the presence of co-administered antibiotics, and they may also transmit the antibiotic-resistance gene...

01 April 2024 8,043 2 View