Is there a protocol for determining intracellular ATP concentrations without using commercial kits. Please suggest.
Hi
Below article and url gives the information about protocol which you asked.
Intracellular ATP Assay of Live Cells Using PTD-Conjugated Luciferase. Sensors 2012, 12, 15628-15637; doi:10.3390/s121115628
http://www.hopkinsmedicine.org/institute_basic_biomedical_sciences/research_centers/metabolism_obesity_research/protocols/pdfs/B3a_Measurement_of_Intracellular_ATP.pdf
Hope this helps
Sreedhar
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