ATP measurements in mammalian cells.

17 May 2017 1 462 Report

Is there a protocol for determining intracellular ATP concentrations without using commercial kits. Please suggest.

Below article and url gives the information about protocol which you asked.

Intracellular ATP Assay of Live Cells Using PTD-Conjugated Luciferase. Sensors 2012, 12, 15628-15637; doi:10.3390/s121115628

http://www.hopkinsmedicine.org/institute_basic_biomedical_sciences/research_centers/metabolism_obesity_research/protocols/pdfs/B3a_Measurement_of_Intracellular_ATP.pdf

Hope this helps

Sreedhar

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