Array-CGH in WG-amplified DNA?

Constantin Polychronakos @Constantin-Polychronakos

03 October 2013 0 9K Report

I need to evaluate mouse DNA for copy-number changes in sample pairs (germline-tumour) with very small tumour samples (50,00-100,000 cells). The lowest amount of DNA my core facility can use for the Agilent CGH array is 500 ng. Even with 100% recovery, I will not be able to get this amount from all my samples. Whole-genome amplification is one possibility. Has anyone got experience with CGH arrays (Agilent 4 x 180k) after WGA?

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