What all thing we should keep in mind while selecting a solvent for hydrothermal reaction in a 50 ml autoclave?

I am going to use a 50 ml teflon lined autoclave for doing hydrothermal synthesis and I am deeply concerned about its safety. The pressure limit inside the autoclave is 3MPa and temperature...

14 February 2018 7,313 3 View

Are there any distinct morphological characters by which Anopheles subpictus species B can be distinguished from other sibling speices?

The available morphological character for distinguishing adults of species B is the lengths of apical white band and subapical dark band of female palpi. However, there are reports of variations...

25 January 2017 1,400 4 View

Is it possible to isolate/detect Japanese Encephalitis virus from Bird droppings?

Migratory birds are likely to transport JE viruses from an endemic area to a non-endemic area. Screening their blood would be the best method to detect JE virus in them. However, it is not...

01 April 2015 8,156 12 View

What is the current status of the Zika virus in Asia?

26 February 2014 7,707 3 View

Does anybody know various theories on the origin and evolution of insects?

In our entomology syllabus one of the components is origin and evolution of insects-various theories. Can anybody suggest a suitable book/paper dealing with this issue?

25 November 2013 4,229 3 View

Can anybody identify the species?

It was found inside an unopened packet of Killer Zone trilogy CD for PS3 brought from the US.

22 November 2013 9,553 14 View

T-test as a statistic for the analysis of fMRI data - can anyone help?

Why are t-tests preferred over other statistical tests for the analysis of fMRI data?

05 October 2013 8,594 8 View

How does matter get its mass ?

By interaction with Higgs field?

08 August 2012 5,331 2 View

What is the total number direct product groups of the form Zn1*Zn2*..... isomorphic to Zn? Here n1*n2*.....=n.

Every cyclic group is isomorphic to Zn. Now what is the total number of isomorphic direct product groups of the form Zn1*Zn2*Zn3*........Where n1*n2*n3*.....=n. It is known that (Zp*Zq) is cyclic...

16 November 2011 5,242 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

Multicloning in pRMC2?

Hi all, I want to express several genes in S.aureus by using the pRMC2 expression vector. I want to try to subclone some genes already cloned into pRMC2 (and their own tetO promoter) and put them...

01 February 2021 5,108 3 View

Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

I want to generate stable Arabidopsis transgenic lines expressing my protein of interest fused with GFP/YFP under the control of its native promoter. I want this for localization studies. Please...

23 January 2021 2,790 3 View

Why is there no fluorescence if GFP-expressing vector is present and sequence is correct?

We recently constructed a plasmid vector with a high-copy number ori, kanamycin resistance, and eGFP under control of the lac promoter but lacking the lac operator. However, the cells are not...

19 January 2021 7,834 2 View

Can anyone please explain about the renaturation of two different subunit of recombinant protein?

Thanks in advance to everyone who answers my question. I want to raise a recombinant N-methylhydantoinase protein by pET expression vector system. The protein is a tetramer of two identical small...

18 January 2021 1,026 2 View

Can anyone recommend utilizing the plasmid extraction kit for extraction of the circular DNA genome of viruses associated with insect vectors?

Dear Colleagues, I am planning to detect and discover DNA viruses particularly those with a circular genome and transmitted by insect vectors. However, I am confused about selecting the best...

10 January 2021 9,534 4 View

Can somebody recommend a dual expression vector?

09 January 2021 2,679 2 View