16 Questions 13 Answers 0 Followers
Questions related from Simon Butler
Im working with DNA origami and I curious if it is possible to recover the staple strands from an agarose seperation of DNA structures and their components
06 September 2019 5,264 3 View
Hi all My DNA origami sample goes into a redsafe stained gel. I seperate the sample into species/bands containing the scaffold strand, the staples and the DNA origami. All bands appear...
04 March 2019 3,920 3 View
Hi Can magnessium chloride be used to adhere DNA origamis to TEM slides in the same way it does mica? Im very excited to get new images but im very unfamiliar with the process. Some papers dont...
19 January 2019 8,586 0 View
Hi Id like to try creating some quite high concentration DNA origami solutions. Using PEG precipitation seems like a really good method but Im unsure if I should look for a pellet after the...
29 October 2018 7,121 1 View
Hi Ive bought gold nanoparticles in the past but feel a higher concentration would be interesting to work with than is currently feasible for me to buy commercially. If I purchased a gram of...
04 June 2018 6,662 8 View
Hi Im functionalising gold nanoparticles with thiolated oligos. Part of all (Or all that Im aware) protocols require spinning the final solution down at 13,300 rpm for 20 minute cycles to...
22 November 2017 5,741 3 View
I thought itd be nice to run a gel contrasting gold nanoparticles, dna functionalised gold nanoparticles and the dna used to functionalise the gold nanoparticles. This would effectively provide 2...
16 October 2017 6,831 6 View
Im trying to identify good protocols. I get very faint bands. I anneal at a 10:1 ratio of staples:scaffold. I run a 1% gel at 70 volts in 1x TBE buffer using a 10000x sybrsafe green stain. I use...
20 September 2017 6,359 1 View
Can a PCR purification column be used in this manner? Thanks
19 September 2017 7,930 3 View
I want to functionalise thiolated oligonucleotides with gold nanoparticles. To this I need to adjust the ph of the solution to 3, add Na+, mix, then bring the solution back to 7. I cant be sure...
07 September 2017 5,697 3 View
Hi If i wanted to seperate a DNA origami of 7249 base pairs size, conformed into a rigid hollow, wire frame sphere, would I need a device with a different molecular weight cut off than a standard...
30 August 2017 9,250 3 View
Hi I have a good paper which suggests DNA origami will break down and should be imaged as soon as possible once fixed on a substrate. Would anyone have any specific advice about DNA origami life...
07 June 2017 5,193 1 View
Hi While investigating if recovery of larger samples of a DNA origami annealing reaction is possible with my current gel electrophoresis set up I discovered that according to thermo...
25 May 2017 9,365 3 View
Hi I am having trouble accurately finding the refractive index of a structure I simulate in HFSS. I have been using chapter 3 of optical metamaterials (Wenshan Cai Vladimir Shalaev) to aid me in...
03 April 2017 4,804 2 View
Hi I'm not having any success (Nothing visible on SEM) binding amine bound 1.4nm nanogold to DNA origami structures. I'm currently using a 100Kd protein concentration column, where origami and...
14 March 2017 1,859 3 View
I'm just trying to create nonlinear DNA origami constructs. I've been using caDNAno but its unsuitable for the creation of truly curved shapes as opposed to pseudo shapes created through a...
04 January 2017 1,244 2 View
