I revived 2 skov3 cell viles together in one flask. These were stored in -80 for more than 6 months. The cells grew like this. It seems they are stressed. But im not understanding what is the reason of stress? can anyone help
I have been doing qpcr for an year now but everytime I am getting errors. Either positive control cq values will vary or there will be no amolification. The knockdown genes are showing increased...
07 June 2024 7,292 1 View
I had mammalian cancer cell line cultured and fixed for confocal imaging. While observing I found these particles around cell nuclei stained with dapi. I checked the main culture for bacteria but...
15 May 2024 9,102 2 View
I had mammalian cancer cells cultuted and fixed on confocal slides. I could see these small small bodies stained with dapi. The main culture flask doesnt show any bacterial growth. Is this...
15 May 2024 6,040 3 View
I was running sds page 8% gel and it started running tilted(see the picture attached). Can anyone tell what can be the reason behind gel running like this?
17 January 2024 7,779 0 View
I revived 2 skov3 cell lines together in a t-25 flask. These were stored in -80 for more than 6 months. Their morphology is changed. They look stressed. But can anyone what can be the cause of stress?
22 December 2023 6,799 0 View
theoretical framework ?
28 November 2023 7,396 2 View
theoretical framework and conceptual framework?
28 November 2023 9,306 0 View
I am trying create a mutant plasmid using DpnI digestion. I had put pcr using the mutant primers. But then as I could not do immediate transformation, I kept it to store in -20 degree. Now if I...
08 September 2023 3,621 1 View
I am trying to develop my western blot using an antibody targeting a 17kda protein. It is binding non-specifically and not giving any band(like in the photo). What does it mean is happening? How...
22 August 2023 3,472 4 View
I tried to revive a2780 cells cryopreserved in -80 degrees. Its been more than 12 hours and its still not attaching. What could possibly be happening? What should I do?
21 August 2023 848 4 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...
02 August 2024 3,174 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.
25 July 2024 9,976 3 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...
15 July 2024 9,975 1 View