Are there suggestions on how to use sonication in order to lyse yeast cells?

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How to calculate mass of reagents based on desired pH and molarity?

Dear all, I am looking for guidance relating to a formula to calculate mass of reagents based on the desired pH and molarity. Is there a formula for this? I am looking to create, and justify with...

28 March 2024 7,457 1 View

How to write an abstract for research?

Abstract

16 March 2024 2,612 2 View

Building Services Enginnering dissertation topics to include zero carbon?

I am researching topics for my dissertation this year so any ideas welcome. Thanks.

26 September 2023 833 0 View

What are current innovations in newspaper distribution models?

I am doing a systems and processes assignment as part of my MBA looking at a case study of newspaper distribution in South Africa. I am keen to see research studies on newspaper distribution...

09 July 2023 4,623 0 View

How to go about problems launching autodock tools 1.5.6 and 1.5.7?

I'm a windows 11 user and I am having problems launching autodock tools 1.5.6 and 1.5.7. The GUI will just launch up to 6-8% and then it will crash and will not continue opening. I already tried...

27 April 2023 1,430 4 View

Redocking in Autodock Vina can I choose any of the conformation as long as its RMSD compared to the original cocrystallized ligand is less than 2?

Hi, I have been trying to redock the cocrystallized ligand of the PPARG protein with PDB ID 4yt1. The best conformation (best binding energy) has a high RMSD relative to the original...

31 July 2022 4,260 6 View

Any test suggestion for 10 years annual data causal relationship?

Hi all, I'm doing fyp with the title of the determinant of healthcare expenditure in 2011-2020. Here are my variables: government financing, gdp, total population. first model is: healthcare...

05 May 2022 5,087 4 View

Methods for Lattice Boltzmann Modelling of cements?

Hi all, I have some x-ray tomography images of cements and I am interested in modelling their permeability using Lattice Boltzmann Modelling. Does anyone know of any code to simulate flow...

03 May 2022 4,945 3 View

Asking about Antibody conjugation onto surface of NH2 modified magnetic nanoparticles?

i am asking about conjugate monoclonal antibody to the surface of NH2 modified MNP i have 2 choices the first using NHS and EDC to activate the Ab first is it safe for antibody activity? the...

03 April 2022 7,534 4 View

Reporting binding energies (kcal/mol) obtained using Autodock Vina?

I would like to ask if I can report the values of binding energies obtained using autodock Vina as mean +/- standard deviation? I docked the ligands in the receptor 5 times (5 trials). Thank you!

10 November 2021 3,934 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View