Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Is there an interpretation for Lineweaver-Burk plot which linear regression lines intersect themselves on quadrant I?

Lineweaver-Burk replots for enzyme inhibition prints regression lines intersecting themselves on quadrant I. The interpretations for competitive, noncompetitive, or uncompetitive inhibition does...

24 November 2020 7,753 3 View

What RNase-free solution can I use to transport fresh mice brains?

I will be having freshly isolated mice brains shipped. However, these brains will be eventually used for RNA isolation and immunofluorescence staining, so it is critical that I have them delivered...

07 October 2020 1,343 3 View

PRECIPITATION MODELING OF V CARBIDE IN Mn STEEL?

HELLO DEAR COLLEGUES, Is there anybody who have acces to MATCALC software that help me modelling the precipitation phenomena in a medium Mn steel microalloyed with 0.15%wt. of V? The expected...

05 October 2020 5,914 3 View

Keeping article quality constant, why do articles from high impact journals tend to receive more citations?

I have realised that papers that I published in relatively higher impact factor journals tend to receive more citations than the others. But why?

28 June 2020 3,283 10 View

Monitoring ATP binding to proteins: TNP-ATP or MANT-ATP?

Hi all, I'm interested on monitoring ATP binding to a protein of interest using fluorescent ATP analogs, such as TNP or MANT-ATP. Do you have any experience with them? Which one is the best...

03 May 2020 3,993 5 View

Can the origin of the cognitive constructs that characterize us be produced by the coevolution of innate human cognitive capacities?

We know that the cognitive capacities that most characterize us are theoretically constructs, that is, non-observational concepts that do not have a concrete existence similar to the physical...

19 March 2020 2,427 3 View

Using VR for recognition and regulation training?

Looking for information on training emotions using VR technology with psychology practices. Any links/help appreciated

07 September 2019 9,830 3 View

Haemotoxylin and Eosin [H&E] weak Eosin stain, how to get rid of the glass cover and which staining step to re-do?

Hi everyone! I tried my first H&E stain with one of the kits I ordered [Vector Labs, cat.no H-3502], and I stained all my slides. However, when I imaged the sections, there was no pink stain only...

25 August 2019 6,168 3 View

There is a way to know at a glance if graphite is exfoliated in graphene, being in solution?

06 March 2019 4,344 5 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Best excel chart to compare algorithms' running time?

Hi I want to compare the running time of robotic path planning algorithms. The grid size is 8X8. I have 100 scenarios of start and goal positions.. Each scenario is run in three obstacles ratio...

22 February 2021 621 1 View

What are the potential shell well and atomic solvation parameters for the boron atom?

I have a protein-ligand docking project. The ligand contains boron atoms that are not detected by Autodock software. To solve this problem, it is necessary to introduce the boron atom to...

19 February 2021 1,236 1 View

How to open an old ".MTS" file containing spectroscopic data?

Hi supreme hive-mind of the internet, we have some old *.MTS files that are supposed to contain spectroscopic data, probably some coordinates and a long list of single line spectra. Basically,...

16 February 2021 9,385 2 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

How to check if the PMF profile obtained from ABF simulation is correct?

I am doing ABF simulations to calculate ligand binding enegy. The PMF profile that I get using single long ABF simulation is attached as pmf_total.png. When I do this ABF calculation with 10...

10 February 2021 4,170 2 View

What is the Greedy windowed algorithm description?

Hi Dear Ph. D., I have carefully read your paper on anomaly detection in smart meters. Unfortunately, the research does not decribe the greedy windowed algorithm, neither its complexity, nor the...

10 February 2021 2,462 3 View