From the literature I understand that TRP channels regulate Ca2+ signalling in cardiomyocytes and fibroblasts. I want to perform immunocytochemical analysis of TRP channels on cultured cells or western blot analysis on heart tissue lysates. There are some TRP channels that have been described previously (TRPC3, TRPC6,7, TRPV1-4, TRPM2, TRPM7 and TRPA1). But is there a high-level of expression of any of these isoforms and if so, is there a reliable antibody against it?
Thanks in advance.
Hello,
I just stumbled on your question and hope my answer would still do you good after this time!
Cardiomyocytes express a whole array of TRP channels; you can check all the work done by Lutz Birnbaumer, Jeffery Molkentin, David Beech, Jessica Sabourin and others several years ago.
As for cardiac fibroblasts, they mainly express TRPC3 and TRPM7; you can check my paper: https://pubmed.ncbi.nlm.nih.gov/30318928/
as well as all the work of Motohiro Nishida.
Good luck
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