Krishna Chander Sridhar

10 Questions 9 Answers 0 Followers

Questions related from Krishna Chander Sridhar

Is the ratio of Th cells to Tregs (CD3/CD25) a good indicator of inflammation in human tissues?

I came across some recent articles that suggest that the ratio of Th17 cells to T reg cells is important indicator of inflammation and autoimmunity. Would it make sense to use the markers CD3 (pan...

27 August 2021 6,918 2 View

Are there reliable antibodies against TRP channels in cardiac cells ?

From the literature I understand that TRP channels regulate Ca2+ signalling in cardiomyocytes and fibroblasts. I want to perform immunocytochemical analysis of TRP channels on cultured cells or...

08 May 2020 6,989 1 View

Are gap junction proteins (Cx43/Cx40/Cx45) expressed in prenatal rat hearts?

I have tried western blot and immunocytochemistry for connexins (40,43,45) which are said to be predominant connexin isoforms in mammalian cardiomyocytes. However, there aren't any traces of them...

06 March 2019 480 2 View

Can someone suggest some literature on efflux mechanisms of excess intracellular calcium in cardiomyocytes?

I am studying the physiological implications of excess calcium in cardiomyocytes and how the cells attain homeostasis in such conditions.

08 June 2018 939 2 View

Is it possible to study the calcium diffusion through gap junctions in cardiomyocytes?

I am interested to study the calcium diffusion along the cardiomyocytes through gap junctions. So I would like to know the dynamics of calcium diffusion. Can someone help me with it? Thanks

04 September 2017 4,662 3 View

Can someone enlighten me about the recent research focus in the field of gravitational biology?

Although it is not my field of study, i am interested atleast to know what is happening in gravitational biology. I have come across some research focusing on plants growing in hyper gravity,...

04 July 2017 4,119 2 View

Is it necessary to centrifuge the cardiomyocytes after loading with Fluo-4 AM for 20 minutes?

If i centrifuge the cells after Fluo4 loading process I feel that the cells are stressed and will not contract as good as without centrifugation. Thanks in advance

19 June 2017 9,005 10 View

Is there a standard working protocol for immunostaining of connexin43/40/45 in rat cardiac cells?

Lately I have been trying to perform immunofluorescence staining for connexins in primary rat cardiac cells but I donot visualize connexin gap junctions at all in my samples. I compared it with...

05 May 2017 580 2 View

Can someone enlighten me with the elasticity of fibronectin coated high precision glass cover slips(170 nm +/- 5 nm #1.5 type)?

I wanted to make a primary cell culture on glass substrate, specifically the high-precision 170 nm +/- 5 nm thick. if I know the elasticity (in KPa or GPa) it would be better for my experiments. I...

07 November 2016 1,711 0 View

Hi fellow researchers. I isolated neonatal rat cardiomyocytes for primary culture studies. But only few clusters beat in culture. Whats the reason?

I suspect that it could be due to the loss of pacemaker cells during the isolation or is it due to the culture conditions.

15 June 2016 3,695 3 View