I am looking for extra small vials for HPLC. Standard vials with inserts have usually a volume of 150 or 200 microliter and a minimum volume of 10 microliters. For an application I would like to have single vials with a volume below 10 microliters. perfect would be a volume of 5 microliters and a residual volume of
try this
http://www.sigmaaldrich.com/catalog/product/supelco/27423?lang=en®ion=US
Thanks,
Are these products (Waters and Sigma) really valid for volumes below 10 Microliters?
To my experiance are both systems valid down to 20 maybe 10 microlitest but not below.
For my experiment I have about 5 microliter of sample (can't dilute much more) and I would like to inject about 1 Microliter for each smaple (3 Injections). therfore I need the smallest available.
Phenomenex offer limited volume speciality vials and conical bottom inserts with residual volumes down to
Yes, you can try this link:
https://www.sun-sri.com/products/overview_av.aspx
I sort of have a similar issue, though mine is with a reagent -- it's sensitive to light and oxygen, so I want to minimize the reagent volume in each aliquot. When in use, I only use 0.5uL for each sample (usually no more than 20 samples), so there is a lot of reagent waste. I looked for a similarly small-volume insert or vial to minimize the interaction with oxygen upon being pierced. I found an insert from a supplier that *said* it held 50uL, but they were only counting the little bulb at the bottom, not the entire volume of the insert. So instead, I currently use a 100uL insert, filled only about 1/3 full, and the extra volume in the entire vial is filled with nitrogen. Your question made me think of the dimensions of 10uL volume. This is the result.
There is a problem with the math of the basic measurements -- I did a quick calculation using reference to 10ga, 20ga, and 30ga needles (I'm not sure the width of your autosampler needle) using Wikipedia as a source for width of these gauges. If the vial were to hold a maximum of 10uL, a 10ga needle could drop into the vial only 1.10mm deep (20ga: 15.4mm; 30ga: 131mm). Then the total volume of pickup at a maximum (unless drawing the sample while descending into the vial) would be 6.25uL (20ga: 4.41uL; 30ga: 2.61uL). At such a low volume, you would have to take into account the volume of the metal of the needle. Though it's a trade off: the finer the gauge of the needle, the softer it will become, and less able to pierce a vial septa.
The only other idea (if you want to be a bit creative) is to get short vials and lose them at the bottom of the autosampler well(s), but being short would hold a smaller volume. AND since it's at the bottom already, it would allow for the full extension of the needle.
Consider keeping the trays, covering/washing/marking the used wells, and use other spots on the tray for the new sample set.
Hope that helps give some perspective.
(sorry for the lengthy post and the 4-year delay)
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